Improved DNA Probe Detection of Listeria monocytogenes in Enrichment Culture After Physical-Chemical Fractionation
نویسندگان
چکیده
منابع مشابه
Rapid quantitative detection of Listeria monocytogenes in chicken using direct and combined enrichment/qPCR method
Listeria monocytogenes is a species of foodborne pathogen often related to foods, such as poultry, ready-to-eat products, fruits, and vegetables. The culture method is a standard procedure for the detection of bacteria in food products. The real-time quantitative PCR (qPCR) technique can be used for the quantification of foodborne pathogens. The current research was aimed to assess...
متن کاملRapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR.
A rapid method for the detection of Listeria monocytogenes in foods combining culture enrichment and real-time PCR was compared to the ISO 11290-1 standard method. The culture enrichment component of the rapid method is based on the ISO standard and includes 24h incubation in half-Fraser broth, 4h incubation in Fraser broth followed by DNA extraction and real-time PCR detection of the ssrA gene...
متن کاملشناسایی لیستریا مونوسیتوژنز در شیر توسط واکنش زنجیره ای پلی مراز
Background: Listeria monocytogenes may be the cause of meningitis and sepsis. It is transmitted via food products. Precise detection of this organism would undoubtedly play a significant role in prevention of this infection. Materials and methods: Polymerase chain reaction method was developed for detection of Listeria monocytogenes in milk samples after enrichment culture. It consists of cult...
متن کاملDetection of hemolytic Listeria monocytogenes by using DNA colony hybridization.
A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized w...
متن کاملOne-step Dna Extraction for Detection of Listeria Monocytogenes
Conventional routine detection of Listeria monocytogenes in food products requires at least 7 days. Thus, the rapid technology for detection of L. monocytogenes is of the utmost importance and urgent necessity. This study aims to develop the rapid DNA extraction for detection of L. monocytogenes by using PCR assay. After DNA extraction of L. monocytogenes, three methods including the deionized ...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2006
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/89.1.172